IdeZ protease Bulk
Similar to IdeS protease, IdeS specifically cleaves peptide bonds below the hinge region of IgG to form Fc and F(ab’)2 fragments. IdeS cleaves all subclasses of human IgG1-4, rabbit, sheep, monkey, humanized and chimeric IgGs, and Fc-fusion proteins. IdeZ also cleaves mouse IgG2a and IgG3 more efficiently than IdeS. Engineered with His tag, it can be removed by IMAC.
IdeZ IgG cleavage site: CPAPELLG*GPSVF
Unit definition:One unit is defined as the amount of enzyme required to digest >95% of 1 ug of human IgG in 30 minutes at 37°C.
Purity: >90% by SDS-PAGE gel
Formulation: 50 mM Phosphate buffer pH 6.6, 150 mM NaCl
Product Source: Cloned from Streptococcus equi subspecies zooepidemicus and expressed in E. coli.
Protocol for IgG cleavage
1. Substrate concentration should be around 0.5-1mg/mL.
2. Around pH 6.8, add IdeZ to substrate and incubate at 37° for at least 30 minutes.
Reference:
1. Lannergård, Jonas, and Bengt Guss. "IdeE, an IgG-endopeptidase of Streptococcus equi ssp. equi." FEMS microbiology letters 262.2 (2006): 230-235.
| Cat No.： | PT-EZ-IdeZ-B |
| Product Name： | IdeZ protease Bulk |
| Type： | Protein |
| Property： | Purified recombinant protein |
| Origin： | |
| Source： | E.coli |
| Application： | FOR LABORATORY RESEARCH USE ONLY. |
| Formulation： | |
| Purity： | >90% by SDS-PAGE gel |
| Storage： | -20 C. Avoid repeated freeze-thaw cycles. |
| Limitation： | FOR LABORATORY RESEARCH USE ONLY. |
| Note： |